Difference between revisions of "Part:BBa K1724001"
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<partinfo>BBa_K1724001 short</partinfo> | <partinfo>BBa_K1724001 short</partinfo> | ||
| − | The wild type | + | The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.[1]That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique. |
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| + | The quantification data of MerR/CadA operon can be seen in <html><a href="https://parts.igem.org/Part:BBa_K1724000" target="_blank">BBa_K194001</a> | ||
| + | == Reference == | ||
| + | 1.Hakkila KM, Nikander PA, Junttila SM, Lamminmaki UJ, Virta MP: Cd-Specific Mutants of Mercury-Sensing Regulatory Protein MerR, Generated by Directed Evolution. Applied and environmental microbiology 2011, 77(17):6215-6224. | ||
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Revision as of 07:51, 15 September 2015
RBS+CsgA
The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.[1]That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.
The quantification data of MerR/CadA operon can be seen in BBa_K194001
== Reference ==
1.Hakkila KM, Nikander PA, Junttila SM, Lamminmaki UJ, Virta MP: Cd-Specific Mutants of Mercury-Sensing Regulatory Protein MerR, Generated by Directed Evolution. Applied and environmental microbiology 2011, 77(17):6215-6224.
Sequence and Features