Difference between revisions of "Part:BBa K1632013:Experience"
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All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br> | All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br> | ||
| − | A. PBAD/araC_FimE(6A1) + | + | A. PBAD/araC_FimE(6A1) +fim switch[default ON]_gfp(3K3) <br> |
| − | B. PBAD/araC_FimE(6A1) + | + | B. PBAD/araC_FimE(6A1) +fim switch[default ON]_gfp(3K3) <br> |
| − | C. rbs_M256IcysE(6A1) + | + | C. rbs_M256IcysE(6A1) + fim switch[default ON]_gfp(3K3) …positive control 1<br> |
| − | D. rbs_M256IcysE(6A1) + | + | D. rbs_M256IcysE(6A1) + fim switch[default OFF]_gfp(3K3) …negative control 1<br> |
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br> | E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br> | ||
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br> | F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br> | ||
Revision as of 07:10, 15 September 2015
PBAD/araC_rbs_fimE(wild-type)
Contents
Materials and Methods
1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.
A. PBAD/araC_FimE(6A1) +fim switch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(6A1) +fim switch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + fim switch[default ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + fim switch[default OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2
2. Assay protocol