Difference between revisions of "Part:BBa K1796006:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1796006 short</partinfo>
 
<partinfo>BBa_K1796006 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
We put it in a pET-28b plasmid to express it.
 
We put it in a pET-28b plasmid to express it.
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The part contains a sequence of 21bp possible RBS region.
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===Source===
 
===Source===
  
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NifH(BBa_K1796006) is taken from genomic sequence of Paenibacillus sp. WLY78, we apply promotions on the sequences and synthesised it.
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NifH(BBa_K1796006) is taken from genomic sequence of ''Paenibacillus sp.''WLY78, we apply promotions on the sequences and synthesised it.
  
 
===References===
 
===References===
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[1]Wang L, Liu Z, Zhao D, Liu X, et al.(2013) A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli, PLoS Genet 9(10):e1003865.

Latest revision as of 06:44, 15 September 2015

Dinitrogenase reductase(nifH)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We put it in a pET-28b plasmid to express it. The part contains a sequence of 21bp possible RBS region.

Source

NifH(BBa_K1796006) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References

[1]Wang L, Liu Z, Zhao D, Liu X, et al.(2013) A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli, PLoS Genet 9(10):e1003865.