Difference between revisions of "Part:BBa K1744002:Design"
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| − | + | ===Design notes=== | |
The killswitch is induced by arabinose. Since arabinose induction is highly dependent on cell metabolism, make sure that the cells will keep their metabolism toward glucose by adding glucose to the medium. To induce the toxin, both in genomic context or in plasmidic context, use arabinose. | The killswitch is induced by arabinose. Since arabinose induction is highly dependent on cell metabolism, make sure that the cells will keep their metabolism toward glucose by adding glucose to the medium. To induce the toxin, both in genomic context or in plasmidic context, use arabinose. | ||
| − | + | ===Source=== | |
The part was constructed using araC and PBAD of pBAD30 (commercially available), a synthetic RBS from Mutalik et al. (2013), the toxin vcrx028 from the conjugative plasmid pVCR94, P1U8, a strong constitutive promoter and a strong RBS from Mutalik et al. (Nature 2013), amilCP, a chromoprotein isolated from Acropora millepora that was BioBrick adapted in E.coli by a previous IGEM team (see BBa_K592009) and KanR (Aph(3’)-I (aminoglycoside | The part was constructed using araC and PBAD of pBAD30 (commercially available), a synthetic RBS from Mutalik et al. (2013), the toxin vcrx028 from the conjugative plasmid pVCR94, P1U8, a strong constitutive promoter and a strong RBS from Mutalik et al. (Nature 2013), amilCP, a chromoprotein isolated from Acropora millepora that was BioBrick adapted in E.coli by a previous IGEM team (see BBa_K592009) and KanR (Aph(3’)-I (aminoglycoside | ||
| − | + | ===Reference=== | |
| + | Vivek K Mutalik, Joao C Guimaraes, Guillaume Cambray, Quynh-Anh Mai1, Marc Juul Christoffersen, Lance Martin, Ayumi Yu, Colin Lam, Cesar Rodriguez, Gaymon Bennett, Jay D Keasling, Drew Endy & Adam P Arkin, Quantitative estimation of activity and quality for collections of functional genetic elements, Nature methods, 10(4), 2013. | ||
Revision as of 00:07, 15 September 2015
Design notes
The killswitch is induced by arabinose. Since arabinose induction is highly dependent on cell metabolism, make sure that the cells will keep their metabolism toward glucose by adding glucose to the medium. To induce the toxin, both in genomic context or in plasmidic context, use arabinose.
Source
The part was constructed using araC and PBAD of pBAD30 (commercially available), a synthetic RBS from Mutalik et al. (2013), the toxin vcrx028 from the conjugative plasmid pVCR94, P1U8, a strong constitutive promoter and a strong RBS from Mutalik et al. (Nature 2013), amilCP, a chromoprotein isolated from Acropora millepora that was BioBrick adapted in E.coli by a previous IGEM team (see BBa_K592009) and KanR (Aph(3’)-I (aminoglycoside
Reference
Vivek K Mutalik, Joao C Guimaraes, Guillaume Cambray, Quynh-Anh Mai1, Marc Juul Christoffersen, Lance Martin, Ayumi Yu, Colin Lam, Cesar Rodriguez, Gaymon Bennett, Jay D Keasling, Drew Endy & Adam P Arkin, Quantitative estimation of activity and quality for collections of functional genetic elements, Nature methods, 10(4), 2013.