Difference between revisions of "Part:BBa K1725080:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
BBa_R0011 with Nhe1 site upstream of promoter (after prefix)
+
BBa_R0011 with Nhe I site upstream of promoter (after prefix)
 
Can easily be synthesised as two oligonucleotides (top and bottom strands) and inserted between EcoR I and Xba I sites of the genes you wish to express under IPTG control.  
 
Can easily be synthesised as two oligonucleotides (top and bottom strands) and inserted between EcoR I and Xba I sites of the genes you wish to express under IPTG control.  
 
The NheI site allows easy diagnostic restriction digest to show that this short sequence has been successfully inserted upstream of your biobrick.
 
The NheI site allows easy diagnostic restriction digest to show that this short sequence has been successfully inserted upstream of your biobrick.

Latest revision as of 21:49, 14 September 2015


Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_R0011 with Nhe I site upstream of promoter (after prefix) Can easily be synthesised as two oligonucleotides (top and bottom strands) and inserted between EcoR I and Xba I sites of the genes you wish to express under IPTG control. The NheI site allows easy diagnostic restriction digest to show that this short sequence has been successfully inserted upstream of your biobrick.

Source

From BBa_R0011.

References