Difference between revisions of "Part:BBa K1725080"
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induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range | induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range | ||
| − | This part is identical to [[BBa_R0011]] except that an Extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest | + | This part is identical to [[Part:BBa_R0011]BBaR0011] except that an Extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest |
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Revision as of 21:42, 14 September 2015
Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site
Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong trasncriptions that can nevertheless be repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]) and induced by IPTG in E. Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range
This part is identical to [[Part:BBa_R0011]BBaR0011] except that an Extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]