Difference between revisions of "Part:BBa K1766008:Design"

 
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===Source===
 
===Source===
  
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EnvZ was isolated from the <i>E.coli</i> genome by ROGER??.
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EnvZ was isolated from <i>E.coli</i> K12.
  
 
===References===
 
===References===

Latest revision as of 19:41, 14 September 2015


EnvZ_V5 osmoregulatory histidine kinase from E.coli.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence includes illegal restirction site EcorI at 315-321 bp. Removed through site-directed mutagenesis exchanging A317G .


Source

EnvZ was isolated from E.coli K12.

References