Difference between revisions of "Part:BBa K1742000:Design"
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===Source=== | ===Source=== | ||
| − | The AsLOV2 fused to a peptide epitope was | + | The AsLOV2 fused to a peptide epitope was synthesized according to the second LOV domain of Avena sativa phototropin 1 (UniprotKB 049003) |
===References=== | ===References=== | ||
Revision as of 18:44, 14 September 2015
Avena sativa LOV2 domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The LOv2 domain was fused to a small peptide epitope to the C-terminus of the J-alpha helix (-KAVDTWV). Depending on the nature of this peptide tag, its ligand ePDZ interacts with different interaction affinities. The AsLOVpep domain also contains a linker 'GGSGGS-' in the N-terminus
Source
The AsLOV2 fused to a peptide epitope was synthesized according to the second LOV domain of Avena sativa phototropin 1 (UniprotKB 049003)