Difference between revisions of "Part:BBa K1744001:Experience"
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This part can be used for recombineering experiment. In this context, amilCP serves us as a plasmidic marker to avoid plasmid background and KanR serves us as a positive recombineering marker to help us select the good recombinants. If the colony are blue, it is plasmidic background, if they are white, then they are good. Both KanR gene and amilCP gene can serve as reporters in several experiments such as plasmid construction, transformation control, recombineering and many more! | This part can be used for recombineering experiment. In this context, amilCP serves us as a plasmidic marker to avoid plasmid background and KanR serves us as a positive recombineering marker to help us select the good recombinants. If the colony are blue, it is plasmidic background, if they are white, then they are good. Both KanR gene and amilCP gene can serve as reporters in several experiments such as plasmid construction, transformation control, recombineering and many more! | ||
| − | [[File: | + | [[File:BBa_K1744001_blue_petri.PNG]] |
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| + | In this previous image, you can see the appearance of colonnies on LB agar when they carry plasmidic or genomic amilCP-KanR cassette. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:33, 14 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1744001
This part can be used for recombineering experiment. In this context, amilCP serves us as a plasmidic marker to avoid plasmid background and KanR serves us as a positive recombineering marker to help us select the good recombinants. If the colony are blue, it is plasmidic background, if they are white, then they are good. Both KanR gene and amilCP gene can serve as reporters in several experiments such as plasmid construction, transformation control, recombineering and many more!
In this previous image, you can see the appearance of colonnies on LB agar when they carry plasmidic or genomic amilCP-KanR cassette.
User Reviews
UNIQef5d392ecfea94c9-partinfo-00000000-QINU UNIQef5d392ecfea94c9-partinfo-00000001-QINU