Difference between revisions of "Part:BBa F2620"
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'''Description'''<br>
 
'''Description'''<br>
 
A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
 
A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
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</div>
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{|align="center" style="padding: 0px" cellspacing="0" cellpadding="0"
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|width='680px' align="left" colspan="4"|'''Characteristics'''
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'''Performance'''<br>
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<center>
 +
{|{{Table}}
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!Experiment*
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!Characteristic*
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!Value*
 
|-
 
|-
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|width='80px'|[[Input Swing|''Input Swing:'']]
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|rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]
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|width='260px'|0.1 - 1000 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
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|[[Input Swing|''Input Swing'']]
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|width='130px' align='left'|[[Resonse time|''L/H Response time:'']]
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|1E-9 - 1E-6 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
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|width='210px' align='left'|9.5 mins (50% of max. output)
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|-
 
|-
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|[[Output Swing|''Output Swing:'']]
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|[[Output Swing|''Output Swing'']]
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|21±3 - 590±9 GFP molecules cfu<sup>-1</sup> s<sup>-1</sup>
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|0±1 - 503±1 GFP molecules cfu<sup>-1</sup> s<sup>-1</sup>
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|align='left'|[[Genetic Stability|''Genetic Stability:'']]
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−
|>92/74 generations (Low/High Input)
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|-
 
|-
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|[[Switch Point|''Switch Point:'']]
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|[[Switch Point|''Switch Point'']]
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|1E-8 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
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|7E-9 ±1E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
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|align='left'|[[Performance Stability|''Performance Stability:'']]
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|>92/74 generations (Low/High Input)
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−
|}
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{|align="center" cellspacing="0" cellpadding="0"
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|width='340px' align="left"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]<br>
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{{Click || image=BC-TransferCurveMatlabTP25-3small.png | link=Part:BBa_F2620:Transfer Function |width=320px | height=230px }}
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|width='340px' align="left"|[[Part:BBa_F2620:Specificity|'''Specificity''']]<br>
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{{Click || image=BC-Specificity-Finalsmall.png | link=Part:BBa_F2620:Specificity |width=320px | height=230px }}
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|-
 
|-
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|align="left"|[[Part:BBa_F2620:Response time|'''Response time''']]<br>
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|rowspan="4"|[[Response time|'''Response time:''']]
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{{Click || image=BC-Latency-3small.png | link=Part:BBa_F2620:Response time |width=320px | height=230px }}
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|''Time from 0 - 50% output''
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|align="left"|[[Part:BBa_F2620:Stability|'''Stability''']]<br><center>
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|9 min
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{{Click || image=BC-Stability-3small.png | link=Part:BBa_F2620:Stability |width=250px | height=230px }}</center>
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|-
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|}
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|''Time from 0 - 90% output''
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<br>
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|27 min
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<div style="padding: 00px; width: 680px">
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|-
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{|align="center" cellspacing="0" cellpadding="0"
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|''Time from 100 - 50% output''
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|width='680px' colspan="4" valign="top"|'''Demand'''
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|# min
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|-
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|''Time from 100 - 5% output''
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|# min
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|-
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|rowspan="2"|[[Part:BBa_F2620:Specificity|'''Specificity''']]
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|''Strong response to''
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|[[3OC6HSL|3OC<sub>6</sub>HSL]], C<sub>6</sub>HSL , C<sub>7</sub>HSL, 3OC<sub>8</sub>HSL, C<sub>8</sub>HSL
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|-
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|''Weak response to''
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|C<sub>4</sub>HSL, C<sub>10</sub>HSL, C<sub>12</sub>HSL
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|-
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|rowspan="2"|[[Part:BBa_F2620:Stability|'''Stability''']]
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|[[Genetic Stability|''Genetic Stability'']]<br>(Low/High Input)
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|>92/74 generations
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|-
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|[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
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|>92/74 generations
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|-
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|rowspan="5"|Demand
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|rowspan="2"|[[Translation Demand|''Translation Demand:'']]<br>(Low/High Input)
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|5040/141600 charged tRNA cfu<sup>-1</sup> s<sup>-1</sup>
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|-
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|336/9440 ribosomes sequestered cfu<sup>-1</sup>
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|-
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|rowspan="2"|[[Transcription Demand|''Transcription Demand:'']]<br>(Low/High Input)
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|(#/#) nucleotides cfu<sup>-1</sup> s<sup>-1</sup>
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|-
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|(#/#) RNAP sequestered cfu<sup>-1</sup>
 
|-
 
|-
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|width='125px' valign="top"| [[Translation Demand|''Translation Demand:'']]<br>(Low/High Input)
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|[[Growth Rate|''Growth Rate'']]<br>(Low/High Input)
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|width='235px' valign="top"| 5040/141600 charged tRNA cfu<sup>-1</sup> s<sup>-1</sup><br>336/9440 ribosomes sequestered cfu<sup>-1</sup>
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|54/59 min doubling time
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|width='105px' valign="top"| [[Growth Rate|''Growth Rate:'']]
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|width='215px' valign="top"| 54/59 min doubling time (Low/High Input)
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|}
 
|}
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</div>
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*Measured by Ania Labno and Barry Canton 2006-2007
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</center>
 
<div style="padding: 00px; width: 680px">
 
<div style="padding: 00px; width: 680px">
 
'''Compatibility'''<br>
 
'''Compatibility'''<br>

Revision as of 22:08, 15 March 2007

Input: 3OC6HSL
Output: PoPS

tetR
R0040		LuxR
I0462		lux pR
R0062
 BBa F2620Icon.png

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.


Performance

Experiment* Characteristic* Value*
Transfer Function Input Swing 1E-9 - 1E-6 M 3OC6HSL, exogenous
Output Swing 0±1 - 503±1 GFP molecules cfu-1 s-1
Switch Point 7E-9 ±1E-9 M 3OC6HSL, exogenous
Response time: Time from 0 - 50% output 9 min
Time from 0 - 90% output 27 min
Time from 100 - 50% output # min
Time from 100 - 5% output # min
Specificity Strong response to 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
Weak response to C4HSL, C10HSL, C12HSL
Stability Genetic Stability
(Low/High Input) 		>92/74 generations
Performance Stability
(Low/High Input) 		>92/74 generations
Demand Translation Demand:
(Low/High Input) 		5040/141600 charged tRNA cfu-1 s-1
336/9440 ribosomes sequestered cfu-1
Transcription Demand:
(Low/High Input) 		(#/#) nucleotides cfu-1 s-1
(#/#) RNAP sequestered cfu-1
Growth Rate
(Low/High Input) 		54/59 min doubling time
  • Measured by Ania Labno and Barry Canton 2006-2007

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.