Difference between revisions of "Part:BBa K283047"

Revision as of 13:07, 14 September 2015

T7 RNA polymerase

This part codes for the T7 RNA polymerase.This part does not pose any biological threat.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1693

Application

Group: FAFU-CHINA

Author: Ruicheng Dai & Changlong Lu

Summary: The inductive effects of IPTG in different concentration and time lengths

IPTG inductive effect

In our project,we built recombinant plasmids and transformed them into brewer yeast to produce dsRNA. Because of the lack of T7 RNA promoter, we transformed a plasmid which contained T7 RNA polymerase gene. And IPTG could induce the expression of T7 RNA polymerase gene. We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect. After the test of IPTG induction,we firmly believed that we could make use of the best IPTG concentration and induction time lengths to get the minimum cost in agriculture.

The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours

The inductive effect of different time length

We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.

@@ Line 17: / Line 17: @@
 <partinfo>BBa_K283047 parameters</partinfo>
 <!-- -->
-<h3>Improvement</h3>
+<h3>Application </h3>
 <h3>Group:   FAFU-CHINA </h3>
 <h3>Author:  Ruicheng Dai & Changlong Lu </h3>
@@ Line 23: / Line 23: @@
 <h3>IPTG inductive effect</h3>
 <p style="margin-right:100px" align="justify">
+In our project,we built recombinant plasmids and transformed them into brewer yeast to produce dsRNA. Because of the lack of T7 RNA promoter, we transformed a plasmid which contained T7 RNA polymerase gene. And IPTG could induce the expression of T7 RNA polymerase gene.
 We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
+After the test of IPTG induction,we firmly believed that we could make use of the best IPTG concentration and induction time lengths to get the minimum cost in agriculture.
 </p>