Difference between revisions of "Part:BBa C0077"

Revision as of 18:29, 12 March 2007

cinR activator from Rhizobium leguminosarum (+LVA)

In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.

Usage and Biology

In Rhizobium leguminosarum, cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in Rhizobium etli, and for the cerRI locus in Rhodobacter sphaeroides. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 528
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_C0077 short</partinfo>
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 ===Usage and Biology===
-In Rhizobium leguminosarum, cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (&lt;200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in Rhizobium etli, and for the cerRI locus in Rhodobacter sphaeroides. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.
+In ''Rhizobium leguminosarum'', cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (&lt;200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in ''Rhizobium etli'', and for the cerRI locus in ''Rhodobacter sphaeroides''. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.
 <span class='h3bb'>Sequence and Features</span>