Difference between revisions of "Part:BBa K1864001:Experience"
 
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<h3>IPTG inductive effect</h3>
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<p style="margin-right:100px" align="justify">
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We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
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</p>
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<br />
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<h3>The inductive effect of different concentration IPTG</h3>
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We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
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[[File:FAFU-CHINA PART (1).png]]
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<h3>The inductive effect of different time length</h3>
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We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
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[[File:FAFU-CHINA PART (2).png]]
 
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Latest revision as of 11:47, 14 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1864001

We built yeast which contains two parts. The first part is CSBV-RdRP (Part:BBa_K1864000) and the second part is T7 RNA polymerase. Meanwhile, IPTG could induce the operation of T7 RNA polymerase. Therefore,we tested the induction results of different IPTG concentration. And we got the data which

User Reviews

UNIQ7f4dbb2bc8e31090-partinfo-00000000-QINU UNIQ7f4dbb2bc8e31090-partinfo-00000001-QINU

IPTG inductive effect

We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.


The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours FAFU-CHINA PART (1).png

The inductive effect of different time length

We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. FAFU-CHINA PART (2).png