Difference between revisions of "Part:BBa K283047"
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<partinfo>BBa_K283047 parameters</partinfo> | <partinfo>BBa_K283047 parameters</partinfo> | ||
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| + | <h3>Improvement</h3> | ||
<h3>Group: FAFU-CHINA </h3> | <h3>Group: FAFU-CHINA </h3> | ||
<h3>Author: Ruicheng Dai & Changlong Lu </h3> | <h3>Author: Ruicheng Dai & Changlong Lu </h3> | ||
Revision as of 11:23, 14 September 2015
T7 RNA polymerase
This part codes for the T7 RNA polymerase.This part does not pose any biological threat.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1693
Improvement
Group: FAFU-CHINA
Author: Ruicheng Dai & Changlong Lu
Summary: The inductive effects of IPTG in different concentration and time lengths
IPTG inductive effect
We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
The inductive effect of different concentration IPTG
We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
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The inductive effect of different time length
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
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