Difference between revisions of "Part:BBa K283047"
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<partinfo>BBa_K283047 parameters</partinfo>
 
<partinfo>BBa_K283047 parameters</partinfo>
 
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<h3>Group:  FAFU-CHINA </h3>
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<h3>Author:  Ruicheng Dai & Changlong Lu </h3>
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<h3>Summary: The inductive effects of IPTG in different concentration and time lengths </h3>
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<h3>IPTG inductive effect</h3>
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<p style="margin-right:100px" align="justify">
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We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
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</p>
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<br />
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<h3>The inductive effect of different concentration IPTG</h3>
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We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
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[[File:FAFU-CHINA PART (1).png]]
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<h3>The inductive effect of different time length</h3>
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We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
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[[File:FAFU-CHINA PART (2).png]]

Revision as of 11:22, 14 September 2015

T7 RNA polymerase

This part codes for the T7 RNA polymerase.This part does not pose any biological threat.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1693


Group: FAFU-CHINA

Author: Ruicheng Dai & Changlong Lu

Summary: The inductive effects of IPTG in different concentration and time lengths

IPTG inductive effect

We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.


The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours FAFU-CHINA PART (1).png

The inductive effect of different time length

We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. FAFU-CHINA PART (2).png