Difference between revisions of "Part:BBa K283047:Experience"
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| − | <h3>Group: FAFU-CHINA | + | <h3>Group: FAFU-CHINA </h3> |
| + | <h3> Author: Ruicheng Dai & Changlong Lu </h3> | ||
| + | <h3>Summary: The inductive effects of IPTG in different concentration and time lengths </h3> | ||
<h3>IPTG inductive effect</h3> | <h3>IPTG inductive effect</h3> | ||
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Revision as of 11:06, 14 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K283047
User Reviews
UNIQce7dda8f9a6bc75b-partinfo-00000000-QINU UNIQce7dda8f9a6bc75b-partinfo-00000001-QINU
Group: FAFU-CHINA
Author: Ruicheng Dai & Changlong Lu
Summary: The inductive effects of IPTG in different concentration and time lengths
IPTG inductive effect
We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
The inductive effect of different concentration IPTG
We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
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The inductive effect of different time length
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
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