Difference between revisions of "Part:BBa K1732005"

Revision as of 06:22, 14 September 2015

J23100-B0034-Renilla-B0015

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 832
Illegal XhoI site found at 641
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 265
1000
COMPATIBLE WITH RFC[1000]

pSB1C3 backbone (part:pSB1C3) with J23100 promoter (BBa_J23100) and RBS (BBa_B0034), Renilla (BBa_52008), and B0015 terminator (BBa_B0015).

Renilla Luciferase, also known as Rluc, is a protein that emits light when coelenterazine is added. Rluc can be used as a reporter to track other proteins or to monitor the activity of a promoter (iGEM 2006_Slovenia).

This sequence has been codon optimized for E. coli and is used to produce optimal amounts of Renilla luciferase so that it can be used to compare the luminescence as measured by standard luminometers as well as other DIY luminometers.

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-Renilla Luciferase, also known as Rluc, is a protein that emits light when coelenterazine is added. Rluc can be used as a reporter to track other proteins or to monitor the activity of a promoter (iGEM 2006_Slovenia).
+pSB1C3 backbone (part:pSB1C3) with J23100 promoter (BBa_J23100) and RBS (BBa_B0034), Renilla (BBa_52008), and B0015 terminator (BBa_B0015).
+Renilla Luciferase, also known as Rluc, is a protein that emits light when coelenterazine is added. Rluc can be used as a reporter to track other proteins or to monitor the activity of a promoter (iGEM 2006_Slovenia).
 [[File:Renilla.jpg]]