Difference between revisions of "Part:BBa K1732005:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1732005 short</partinfo>
 
<partinfo>BBa_K1732005 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Sequence is codon optimized for E. coli
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pSB1C3 backbone (part:pSB1C3) with J23100 promoter (BBa_J23100) and RBS (BBa_B0034), Renilla (BBa_52008), and B0015 terminator (BBa_B0015). 
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 +
Sequence is codon optimized for E. coli which is why the Renilla luciferase sequence differs from the original BBa_52008 part)
  
  
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===Source===
 
===Source===
  
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Sequence designed by Cheryl Telmer via IDT
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Sequence designed by Cheryl Telmer via IDT. Codon optimized Renilla Luciferase to allow optimal productions of Rluc.
  
 
===References===
 
===References===

Latest revision as of 06:21, 14 September 2015

J23100-B0034-Renilla-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 832
    Illegal XhoI site found at 641
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pSB1C3 backbone (part:pSB1C3) with J23100 promoter (BBa_J23100) and RBS (BBa_B0034), Renilla (BBa_52008), and B0015 terminator (BBa_B0015).

Sequence is codon optimized for E. coli which is why the Renilla luciferase sequence differs from the original BBa_52008 part)


Source

Sequence designed by Cheryl Telmer via IDT. Codon optimized Renilla Luciferase to allow optimal productions of Rluc.

References