Difference between revisions of "Part:BBa K1595030:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1595030 short</partinfo> | <partinfo>BBa_K1595030 short</partinfo> | ||
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Use in conjunction with strain-expressing T7 polymerase. | Use in conjunction with strain-expressing T7 polymerase. | ||
| − | + | Cel5a is a stable, detectable protein that can be used to determine expression of our plasmid. | |
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| + | EDA had an internal PstI cutsite that was removed by altering the DNA sequence. | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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| + | EDA: NCBI accession number P0A955 | ||
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| + | Cel5a: Pereira et al. <i>Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima</i>. Journal of Structural Biology. Vol 172(3). Dec 2010. 372-379. | ||
Latest revision as of 04:19, 14 September 2015
EDA linked to cel5a via GSG linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1965
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 641
Illegal NgoMIV site found at 1038
Illegal NgoMIV site found at 1494
Illegal AgeI site found at 1758 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1755
Design Notes
Use in conjunction with strain-expressing T7 polymerase.
Cel5a is a stable, detectable protein that can be used to determine expression of our plasmid.
EDA had an internal PstI cutsite that was removed by altering the DNA sequence.
Source
Synthesize by DNA 2.0
References
EDA: NCBI accession number P0A955
Cel5a: Pereira et al. Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima. Journal of Structural Biology. Vol 172(3). Dec 2010. 372-379.