Difference between revisions of "Part:BBa K1819001:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
−
Because Lcp is secreted by  Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server [(http://www.compgen.org/tools/PRED-TAT)].[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites
+
Because Lcp is secreted by  Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server http://www.compgen.org/tools/PRED-TAT.[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites
  
 
===Source===
 
===Source===

Revision as of 02:57, 14 September 2015

Latex Clearing Protein (Lcp) - rubber degradation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 889
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438


Design Notes

Because Lcp is secreted by Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server http://www.compgen.org/tools/PRED-TAT.[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites

Source

References

1 Bagos PG, Nikolaou EP, Liakopoulos TD, Tsirigos KD. Combined prediction of Tat and Sec signal peptides with Hidden Markov Models. Bioinformatics. 2010; 6(22):2811-2817.