Difference between revisions of "Part:BBa K1595029:Design"

 
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<partinfo>BBa_K1595029 short</partinfo>
 
<partinfo>BBa_K1595029 short</partinfo>
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===Design Notes===
 
===Design Notes===
 +
 
Use in conjunction with strain-expressing T7 polymerase.
 
Use in conjunction with strain-expressing T7 polymerase.
  
 
Added GSG so EDA could easily be linked to it's associated peptide in future use.
 
Added GSG so EDA could easily be linked to it's associated peptide in future use.
 
 
  
 
===Source===
 
===Source===
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===References===
 
===References===
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 +
EDA: NCBI accession number P0A955

Revision as of 02:00, 14 September 2015

EDA and GSG linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 641
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use in conjunction with strain-expressing T7 polymerase.

Added GSG so EDA could easily be linked to it's associated peptide in future use.

Source

Synthesized by DNA 2.0

References

EDA: NCBI accession number P0A955