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| − | ===Materials and Methods===
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| − | <b>1. Construction</b><br>
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| − | All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br>
| + | <strong>Please click [https://parts.igem.org/Part:BBa_K16320013:Experience here]</strong>. (PBAD/AraC-rbs-fimE(Wild Type) Experiment Page) |
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| − | A. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3) <br>
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| − | B. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3) <br>
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| − | C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1<br>
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| − | D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1<br>
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| − | E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br>
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| − | F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br>
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| − | <b>2. Assay protocol</b><br>
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Revision as of 23:17, 13 September 2015
Please click here. (PBAD/AraC-rbs-fimE(Wild Type) Experiment Page)
