Difference between revisions of "Part:BBa K1620007"
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− | SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick <partinfo>BBa_K519010</partinfo> originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed. | + | SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick <partinfo>BBa_K519010</partinfo> originally cloned from ''Synechococcus'' sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in ''E. coli'' improving its growth at a high Cd(II) concentrationwhen compared to wild type ''E. coli''. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed '''G''' -> '''A'''. No residues were changed. |
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− | [[File: Alignment between improved SmtA and BBa_K519010.jpg|450px|thumb|left|'''Figure 1:''' | + | [[File: Alignment between improved SmtA and BBa_K519010.jpg|450px|thumb|left|'''Figure 1:''' Improved SmtA (<partinfo>BBa_K1620007</partinfo>) compared to <partinfo>BBa_K519010</partinfo>; this new sequence is biobrick compatible due the removal of a PstI restriction site previously present in <partinfo>BBa_K519010</partinfo>.]] |
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Revision as of 22:05, 13 September 2015
Synechococcus metallothionenin A (SmtA)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 121
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick BBa_K519010 originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.