Difference between revisions of "Part:BBa K1620001:Design"
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===Source=== | ===Source=== | ||
| − | The promoter element was designed through bioinformatic analysis of 500bp frame upstream genes of operon ZnuABC from E. coli K12 MG1655 genome. Then, it was synthesized by IDT through G-block and further assembled by 3A assembly method. | + | The promoter element was designed through bioinformatic analysis of 500bp frame upstream genes of operon ZnuABC from ''E. coli'' K12 MG1655 genome, as observed in Figure 2. Then, it was synthesized by IDT through G-block and further assembled by 3A assembly method. |
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| + | [[Image:UFSCariGEM2015_Zasp_EcoliGenome.jpg|450px|thumb|center|'''Figure 2:''' Genome of ''E. coli'' K-12 MG1655 showing the operon genes ''znuABC'' and the region of where the Zasp promoter was obtained.]] | ||
===References=== | ===References=== | ||
Revision as of 21:36, 13 September 2015
Promoter element Zasp
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have identified two sequences of this promoter (one in sense strand and another in the antisense strand). In order to turn this sequence more feasible we used a hybrid final sequence. The final sequence was believed to promote for both sites.
Source
The promoter element was designed through bioinformatic analysis of 500bp frame upstream genes of operon ZnuABC from E. coli K12 MG1655 genome, as observed in Figure 2. Then, it was synthesized by IDT through G-block and further assembled by 3A assembly method.
