Difference between revisions of "Part:BBa K1620001"

 
 
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It is a zinc activity sensing promoter element. The regulation of this promoter is Zur dependant and positive in absent of zinc or other divalent metallic cations in medium. The Zur protein binds those cations repressing the expression of the gene downstream Zasp element. In our project, it was used to trigger the expression of killer genes downstream this element. Since ZnuABC activity is extremelly sensitive to low zinc concentrations, we believe that this component will be usefull for other teams in order to its inducible feature and precise functioning.
 
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===Usage and Biology===
 
  
 
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===Usage and Biology===
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===Functional Parameters===
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<partinfo>BBa_K1620001 parameters</partinfo>
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It is a zinc activity sensing promoter element. The regulation of this promoter is Zur dependent and positive in absent of zinc or other divalent metallic cations in medium. The Zur protein binds those cations repressing the expression of the gene downstream Zasp element. In our project, it was used to trigger the expression of killer genes downstream this element. Since ZnuABC activity is extremely sensitive to low zinc concentrations, we believe that this component will be useful for other teams in order to its inducible feature and precise functioning.
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Latest revision as of 21:24, 13 September 2015

Promoter element Zasp

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

It is a zinc activity sensing promoter element. The regulation of this promoter is Zur dependent and positive in absent of zinc or other divalent metallic cations in medium. The Zur protein binds those cations repressing the expression of the gene downstream Zasp element. In our project, it was used to trigger the expression of killer genes downstream this element. Since ZnuABC activity is extremely sensitive to low zinc concentrations, we believe that this component will be useful for other teams in order to its inducible feature and precise functioning.