Difference between revisions of "Part:BBa K1819000:Design"

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<partinfo>BBa_K1819000 short</partinfo>
 
<partinfo>BBa_K1819000 short</partinfo>

Latest revision as of 20:58, 13 September 2015

GFP coding sequence with an N-terminal linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674


Design Notes

As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag, then it should not have any scar.

Source

GFP coding sequence source is BBa_E0040.

References