Difference between revisions of "Part:BBa K1819000:Design"
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GFP coding sequence source is <a href="https://parts.igem.org/Part:BBa_E0040">BBa_E0040</a>. | GFP coding sequence source is <a href="https://parts.igem.org/Part:BBa_E0040">BBa_E0040</a>. | ||
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===References=== | ===References=== | ||
Revision as of 20:34, 13 September 2015
GFP coding sequence with an N-terminal linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 674
Design Notes
As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag, then it should not have any scar.
Source
GFP coding sequence source is BBa_E0040.