Difference between revisions of "Part:BBa K1819000:Design"

 
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===Design Notes===
 
===Design Notes===
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As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag.
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As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag, then it should not have any scar.
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===Source===
 
===Source===

Revision as of 20:28, 13 September 2015


GFP coding sequence with an N-terminal linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674


Design Notes

As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag, then it should not have any scar.

Source

GFP coding sequence source is BBa_E0040 (https://parts.igem.org/Part:BBa_E0040)

References