Difference between revisions of "Part:BBa K1628303"

Latest revision as of 15:55, 13 September 2015

P3-GFP

P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1628303 short</partinfo>
-P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117 and a pSB1C3 backbone. We transformed this plasmid into ''E. coli'' cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.
+P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.
 <!-- Add more about the biology of this part here