Difference between revisions of "Part:BBa K1632013:Experience"
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E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br> | E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br> | ||
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br> | F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br> | ||
| + | [[Image:Tokyo_Tech_FimE_assay.png|thumb|center|600px|<b>Fig. 1. </b>Plasmids]]<br> | ||
<b>2. Assay protocol</b><br> | <b>2. Assay protocol</b><br> | ||
Revision as of 13:41, 13 September 2015
Materials and Methods
1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.
A. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2
2. Assay protocol