Difference between revisions of "Part:BBa K1632013:Experience"

 
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===Materials and Methods===
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<b>1. Construction</b><br>
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All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br>
  
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__NOTOC__
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A. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3) <br>
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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B. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3) <br>
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how you used this part and how it worked out.
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C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1<br>
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D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1<br>
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E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br>
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F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br>
  
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===Applications of BBa_K1632013===
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<b>2. Assay protocol</b><br>
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===User Reviews===
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<!-- Template for a user review
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_K1632013 AddReview number</partinfo>
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<I>Username</I>
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|width='60%' valign='top'|
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Enter the review inofrmation here.
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|};
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<!-- End of the user review template -->
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<!-- DON'T DELETE --><partinfo>BBa_K1632013 EndReviews</partinfo>
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Revision as of 13:34, 13 September 2015

Materials and Methods

1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.

A. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2

2. Assay protocol