Difference between revisions of "Part:BBa K1699005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Designed using Benchling to meet registry standards. Synthesized by IDT. Cloned into pSB1C3 using EcoRI and PstI | + | Designed using Benchling to meet registry standards. Synthesized by IDT. Cloned into pSB1C3 using EcoRI and PstI restriction sites. |
===Source=== | ===Source=== | ||
gMLP from IDT de-novo synthesis. Cloning with U6 to pSBC13 was done by team BGU 2015. | gMLP from IDT de-novo synthesis. Cloning with U6 to pSBC13 was done by team BGU 2015. | ||
Revision as of 11:36, 13 September 2015
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 250
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed using Benchling to meet registry standards. Synthesized by IDT. Cloned into pSB1C3 using EcoRI and PstI restriction sites.
Source
gMLP from IDT de-novo synthesis. Cloning with U6 to pSBC13 was done by team BGU 2015.