Difference between revisions of "Part:BBa K1699003:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
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Cloned into pSBC13 using EcoRI and PstI restriction sites.
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Designed using Benchling to meet registry standards. Synthesized by Syntezza Biosience. Cloned into pSB1C3 using EcoRI and PstI restrictoion sites.
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<br /> F. tagtcatgGAATTCGCGGCCGCTTCTAG
+
−
<br /> R. tgtatactCTGCAGCGGCCGCTAC
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===Source===
 
===Source===

Revision as of 11:09, 13 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed using Benchling to meet registry standards. Synthesized by Syntezza Biosience. Cloned into pSB1C3 using EcoRI and PstI restrictoion sites.

Source

IDT de-novo synthesis.


References

1. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full

2. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas
http://pubs.acs.org/doi/full/10.1021/sb400081r

3. In vivo genome editing using Staphylococcus aureus Cas9.
http://10.1038/nature14299