Difference between revisions of "Part:BBa K1595020:Design"
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EcnB: NCBI source number AGW91308 | EcnB: NCBI source number AGW91308 | ||
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Latest revision as of 06:32, 13 September 2015
MBP with EcnB from Ralstonia pickettii.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1257
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1257
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1257
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1257
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 138
Design Notes
Use in conjunction with strain-expressing T7 polymerase.
Has internal PstI cutsite.
Source
Synthesized by DNA 2.0
References
EcnB: NCBI source number AGW91308
MBP-TEV: BBa_K407015