Difference between revisions of "Part:BBa K1595020:Design"
(→Design Notes) |
(→References) |
||
Line 16: | Line 16: | ||
===References=== | ===References=== | ||
+ | EcnB: NCBI source number AGW91308 | ||
+ | MBP: BBa_K407015 |
Revision as of 06:31, 13 September 2015
MBP with EcnB from Ralstonia pickettii.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1257
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1257
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1257
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1257
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 138
Design Notes
Use in conjunction with strain-expressing T7 polymerase.
Has internal PstI cutsite.
Source
Synthesized by DNA 2.0
References
EcnB: NCBI source number AGW91308 MBP: BBa_K407015