Difference between revisions of "Part:BBa K1595020:Design"

 
(Design Notes)
Line 9: Line 9:
 
Use in conjunction with strain-expressing T7 polymerase.
 
Use in conjunction with strain-expressing T7 polymerase.
  
 
+
Has internal PstI cutsite.
  
 
===Source===
 
===Source===

Revision as of 05:46, 13 September 2015


MBP with EcnB from Ralstonia pickettii.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1257
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


Design Notes

Use in conjunction with strain-expressing T7 polymerase.

Has internal PstI cutsite.

Source

Synthesized by DNA 2.0

References