Difference between revisions of "Part:BBa K1659100:Design"

Latest revision as of 16:23, 12 September 2015

Microcin S, a Class II microcin produced by probiotic E. coli G3/10

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes and Sources

A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography.

We obtained the peptide sequence for MccS from Zschüttig et al's original publication and converted it into a nucleotide sequence using IDT's Codon Optimizer, optimizing based on E. coli K-12's codon table [1].

References

[1] Zschüttig, A., Zimmermann, K., Blom, J., Goesmann, A., Pöhlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1–9. http://doi.org/10.1371/journal.pone.0033351

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1659100 short</partinfo>
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-===Design Notes===
+===Design Notes and Sources===
-N-terminal His tag
-===Source===
+A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography.
+We obtained the peptide sequence for MccS from Zschüttig et al's original publication and converted it into a nucleotide sequence using [https://www.idtdna.com/CodonOpt IDT's Codon Optimizer], optimizing based on ''E. coli'' K-12's codon table [1].
-UniProtKB - H9ZMG7 (H9ZMG7_ECOLX)
 ===References===
 [1] Zschüttig, A., Zimmermann, K., Blom, J., Goesmann, A., Pöhlmann, C., & Gunzer, F. (2012). Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS ONE, 7(3), 1–9. http://doi.org/10.1371/journal.pone.0033351