Difference between revisions of "Part:BBa K1686045"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <p align="justify"> | ||
| + | Curdlan is a bacterial polysaccharide (linear (1->3)-β-glucan) that has been of significant recent interest due to its valuable properties and its bioactivity. | ||
| + | In fact, Curdlan belongs to the class of biological response modifiers that enhance or restore normal immune defenses. For example, it can have antitumor, anti-infective, anti-inflammatory, and anticoagulant activities (see other properties of Curdlan). In particular, this (1->3)-β-glucan can stimulate plants' immune system. | ||
| + | <br>Our project is focused on the production of curdlan for stimulate plant defense system against Downy Mildew. | ||
| + | </p> | ||
| + | <p align="justify"> | ||
| + | The molecular genetics of curdlan production have been investigated in ''Agrobacterium sp.'' ATCC31749. | ||
| + | Studies identified three genes (''crdA'', ''crdS'' and ''crdC'') that are essential for curdlan production | ||
| + | The ''crdASC'' genes occupy a contiguous 4,948 bp region of the genome ( the crdS gene is flanked by the ''crdA'' and ''crdC'' genes). | ||
| + | </p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 15:35, 12 September 2015
crdA gene with codon optimisation for E. coli
CrdA might assist translocation of the nascent polymer of Curdlan across the cytoplasmic membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 399
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 859
Illegal AgeI site found at 828 - 1000COMPATIBLE WITH RFC[1000]