Difference between revisions of "Part:BBa K1604000"

(→‎Usage and Biology)
(→‎Usage and Biology)
 
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To see the characterization results check the part <html><a href="https://parts.igem.org/Part:BBa_K1604010">BBa_K1604010</a></html>.<br>
 
To see the characterization results check the part <html><a href="https://parts.igem.org/Part:BBa_K1604010">BBa_K1604010</a></html>.<br>
  
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Check out our Wiki <html><a>"http://2015.igem.org/Team:UNITN-Trento">UNITN-Trento iGEM 2015</a><br><br></html>
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Check out our Wiki <html><a href="http://2015.igem.org/Team:UNITN-Trento">UNITN-Trento iGEM 2015</a>! </html>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 14:47, 12 September 2015

Strong RBS + Proteorhodopsin

Proteorhodopsin (PR) is a light-powered proton pump. It creates a proton motive force across the membrane that can subsequently power cellular processes, such as ATP synthesis.

Usage and Biology

We improved part BBa_K773002 of Caltech 2012 by adding a strong RBS. We placed BBa_K1604000 under an inducible promoter and we fully characterized proteorhodopsin.
To see the characterization results check the part BBa_K1604010.

Check out our Wiki UNITN-Trento iGEM 2015!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 529
    Illegal XhoI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 666
  • 1000
    COMPATIBLE WITH RFC[1000]