Difference between revisions of "Part:BBa K1632011:Design"

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<partinfo>BBa_K1632011 short</partinfo>
 
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<partinfo>BBa_K1632011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1632011 SequenceAndFeatures</partinfo>
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2006_Caltech FimE(BBa_K137007) didn’t match the nucleotide sequence of fimE of wild type. So, we designed FimE (BBa_K1632011) which completely match the nucleotide sequence of fimE of wild type. Compared with these two parts, two differences were confirmed. First, fimE of 2008_Caltech lacks the nucleotide sequence of N-terminal 15 residues. Second, fimE of 2008_Caltech is different from the nucleotide sequence of C-terminal 2 residues as shown as below.<br>
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2008_Caltech : 5’-...-TAA-TAA-Suffix-3’<br>
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2015_TokyoTech : 5’-...-GTT-TGA-Suffix-3’<br>
  
  

Revision as of 13:29, 12 September 2015

fimE (wild-type)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]



2006_Caltech FimE(BBa_K137007) didn’t match the nucleotide sequence of fimE of wild type. So, we designed FimE (BBa_K1632011) which completely match the nucleotide sequence of fimE of wild type. Compared with these two parts, two differences were confirmed. First, fimE of 2008_Caltech lacks the nucleotide sequence of N-terminal 15 residues. Second, fimE of 2008_Caltech is different from the nucleotide sequence of C-terminal 2 residues as shown as below.
2008_Caltech : 5’-...-TAA-TAA-Suffix-3’
2015_TokyoTech : 5’-...-GTT-TGA-Suffix-3’


Design Notes

sequence confirmed

Materials and Methods

Source

PCR from MG1655

References