Difference between revisions of "Part:BBa K1641024"

 
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<partinfo>BBa_K1641024 short</partinfo>
 
<partinfo>BBa_K1641024 short</partinfo>
  
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This is the reporter of inversion activity of invertase Cre. An up-side-down mcherry gene with RBS is surrounded by a pair of LoxP and controlled by a constructive promoter BBa_J23101. At first stage, due to the meaningless mRNA, red signal cannot be generated, but when Cre exist,it inverts and restore the mcherry gene between two LoxP, the red signal can be generated at once.
 
  
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This device is constructed to measure the real-time dynamics of timing module of Cre in the Micro-timer presented by SYSU-CHINA.
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This is a reporter of invertase activity of Cre for Real-time measurement of dynamics of timing modules by SYSU-CHINA 2015.
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The target sequence LoxP of Cre locates in the pInv-rep, surrounding a mcherry gene which is yet upside-down and transcribed by a constructive promoter. This mcherry-coding sequence can be inverted and restored to 5’ – 3’ direction at the existence of Cre or its EGFP fusion, rendering red signal. By real-time measurement of EGFP and mcherry, we can obtain data of Cre dynamics and simulate this process through modelling.
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For this reporter:
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RTS type: LoxP
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mcherry type: BBa_J06504-BBa_M0052, which is mcherry-ssra(moderately fast)
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Promoter type: BBa_J23100
  
 
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Revision as of 09:51, 12 September 2015

Reporter of Invertase activity of Cre, pInv-rep-100LoxM


This is a reporter of invertase activity of Cre for Real-time measurement of dynamics of timing modules by SYSU-CHINA 2015.

The target sequence LoxP of Cre locates in the pInv-rep, surrounding a mcherry gene which is yet upside-down and transcribed by a constructive promoter. This mcherry-coding sequence can be inverted and restored to 5’ – 3’ direction at the existence of Cre or its EGFP fusion, rendering red signal. By real-time measurement of EGFP and mcherry, we can obtain data of Cre dynamics and simulate this process through modelling.

For this reporter: RTS type: LoxP mcherry type: BBa_J06504-BBa_M0052, which is mcherry-ssra(moderately fast) Promoter type: BBa_J23100

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]