Difference between revisions of "Part:BBa M31502:Design"

(→‎Changes: Where and Why)
(→‎Changes: Where and Why)
Line 1: Line 1:
 
==Changes: Where and Why==
 
==Changes: Where and Why==
−
#bp 814-819: want to remove Gene 10 (-10) promoter binding sequence
+
#bp 415-420: want to remove Gene 10 (-10) promoter binding sequence
 
#*frame = TTG ACA : Leu Thr
 
#*frame = TTG ACA : Leu Thr
 
#**change to = CTG  ACC : Leu Thr
 
#**change to = CTG  ACC : Leu Thr
 +
#Cut gene 2 at HpaI site. HpaI site will be a boundary for section A of M13.1 refactored genome

Revision as of 00:58, 25 February 2007

Changes: Where and Why

  1. bp 415-420: want to remove Gene 10 (-10) promoter binding sequence
    • frame = TTG ACA : Leu Thr
      • change to = CTG ACC : Leu Thr
  2. Cut gene 2 at HpaI site. HpaI site will be a boundary for section A of M13.1 refactored genome