Difference between revisions of "Part:BBa K1669001"

Revision as of 07:42, 12 September 2015

CtfA + His-tag

This part includes the CtfA gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA.

The sequence contains a C-terminal His-tag for purification of the protein using the immobilized-metal affinity chromatography and detected with the anti-His antibodyes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 648
Illegal AgeI site found at 175
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part includes the CtfA gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA.
-The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes.
+The sequence contains a C-terminal His-tag for purification of the protein using the immobilized-metal affinity chromatography and detected with the anti-His antibodyes.
 <!-- Add more about the biology of this part here