Difference between revisions of "Part:BBa K1606029"
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we modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. | we modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. | ||
| + | <h2 align=center>mCherry(restricted)-GFP-GFP</h2> | ||
| + | https://static.igem.org/mediawiki/2015/thumb/9/97/MCherry%28restricted%29-GFP-GFP-PGap-z.JPG/319px-MCherry%28restricted%29-GFP-GFP-PGap-z.JPG | ||
| + | |||
| + | <h2 align=center>101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry</h2> | ||
| + | https://static.igem.org/mediawiki/2015/f/fa/101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG | ||
| + | |||
| + | <h2 align=center>KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry</h2> | ||
| + | https://static.igem.org/mediawiki/2015/0/06/Kumma123metu.PNG | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 00:12, 12 September 2015
Gluten Updated m-cherry reporter gene
we modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment.
mCherry(restricted)-GFP-GFP
101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry
KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 567
Illegal AgeI site found at 679 - 1000COMPATIBLE WITH RFC[1000]