Difference between revisions of "Part:BBa K1606007"
 
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https://static.igem.org/mediawiki/2015/f/fa/101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG
 
https://static.igem.org/mediawiki/2015/f/fa/101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG
  
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<h2 align=center>KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry</h2>
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https://static.igem.org/mediawiki/2015/0/06/Kumma123metu.PNG
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:11, 12 September 2015

Kumamax enzyme that gluten updated m-cherry reporter device

We modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation.


mCherry(restricted)-GFP-GFP

319px-MCherry%28restricted%29-GFP-GFP-PGap-z.JPG

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG

KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry

Kumma123metu.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1397
    Illegal NgoMIV site found at 1670
    Illegal NgoMIV site found at 2198
    Illegal NgoMIV site found at 2444
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
    Illegal AgeI site found at 1722
    Illegal AgeI site found at 2298
    Illegal AgeI site found at 2538
  • 1000
    COMPATIBLE WITH RFC[1000]