Difference between revisions of "Part:BBa K1606007"
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<h2 align=center>101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry</h2>
 
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<img src="https://static.igem.org/mediawiki/2015/f/f9/101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry.JPG">
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/d/d0/Gluten_pqlp.PNG" width="600px">
 
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<h2 align=center>Project Summary</h2>
 
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<p align=center>People with celiac disease have to consume gluten free food because gluten creates an auto-immune response in their body. However, gluten free food is a lot more expensive than normal food. That is why, we decided to find a solution to this problem by taking kumamolisin and putting it in a vector of yeast that has alpha secretion factor then putting the plasmid in yeast. The yeast will produce and secrete kumamolisin enzyme to its external environment and will destroys gluten while the bread continues to be fermented. We also prepared a gluten detection kit using green fluroscent protein in PQLP aminoacid sequence. If kumamolisin cuts the gluten it will have cut GFP as well so there will be no colour but if it does not cut the gluten than it also has not cut GFP so green color will be observed.
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:08, 12 September 2015

Kumamax enzyme that gluten updated m-cherry reporter device

We modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation.


mCherry(restricted)-GFP-GFP-PGap-z

319px-MCherry%28restricted%29-GFP-GFP-PGap-z.JPG

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1397
    Illegal NgoMIV site found at 1670
    Illegal NgoMIV site found at 2198
    Illegal NgoMIV site found at 2444
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
    Illegal AgeI site found at 1722
    Illegal AgeI site found at 2298
    Illegal AgeI site found at 2538
  • 1000
    COMPATIBLE WITH RFC[1000]