Difference between revisions of "Part:BBa K1773020"
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<partinfo>BBa_K1773020 short</partinfo> | <partinfo>BBa_K1773020 short</partinfo> | ||
− | Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is | + | Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and medium RBS site. We wanted to have different expression strengths of Cas3, so we cloned it also to a strong RBS (BBa_K1773019), and a weak RBS (BBa_K1773021). |
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+ | In the I-F type CRISPR-Cas system Cascade complex (BBa_K1773005) distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction. | ||
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Latest revision as of 14:17, 11 September 2015
Cas3 gene with pLux/cI right promoter and Medium RBS
Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and medium RBS site. We wanted to have different expression strengths of Cas3, so we cloned it also to a strong RBS (BBa_K1773019), and a weak RBS (BBa_K1773021).
In the I-F type CRISPR-Cas system Cascade complex (BBa_K1773005) distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2720
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1441
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2404
Illegal AgeI site found at 2777 - 1000COMPATIBLE WITH RFC[1000]