Difference between revisions of "Part:BBa K1604022:Design"
Cridelbianco (Talk | contribs) (→Design Notes) |
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The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR. | The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR. | ||
| − | + | NOTE | |
| + | This device was built using a part from the Cambridge 2009 team . Be careful when operating this part, because the SpEI site in the suffix is missing. The part from Cambridge 2009 had this mistake and so the error was introduced in our part. We realized that after sequencing. Other than that the device works well. | ||
===Source=== | ===Source=== | ||
Latest revision as of 16:58, 10 September 2015
araC-pBAD + β-carotene + J23100 + blh
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 9
Illegal NheI site found at 32 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2041
Illegal BamHI site found at 4082 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
Illegal NgoMIV site found at 3618
Illegal NgoMIV site found at 3748
Illegal AgeI site found at 1876
Illegal AgeI site found at 2833 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1858
Design Notes
The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR.
NOTE This device was built using a part from the Cambridge 2009 team . Be careful when operating this part, because the SpEI site in the suffix is missing. The part from Cambridge 2009 had this mistake and so the error was introduced in our part. We realized that after sequencing. Other than that the device works well.
Source
AraC-pBAD promoter comes from E.coli genome, while ctrEIBY are from Pantoea ananatis (formerly Erwinia uredovora) genome. Blh gene and its promoter are synthetized and codon optimezed by GeneScript.