Difference between revisions of "Part:BBa K1686046"
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| + | A central large and relatively hydrophilic cytoplasmic re- gion of !300 residues situated between TM3 and TM4 carries various conserved motifs, including the UDPGlc substrate-binding and catalytic D,D,D35QxxRW motif | ||
The crdS gene of Agrobacterium sp. strain ATCC31749 encodes the curdlan synthase (CrdS) protein | The crdS gene of Agrobacterium sp. strain ATCC31749 encodes the curdlan synthase (CrdS) protein | ||
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| + | encodes a membrane protein | ||
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| + | Locus I is a putative operon of three genes in which the crdS gene, encoding a protein homologous with family GT- 2 glycosyl transferases (Coutinho and Henrissat, 1999), is flanked by the crdA and crdC genes (Stasinopoulos et al., 1999). The latter two genes encode proteins sharing no significant homology with reported protein sequences, and their roles in curdlan production are unknown. | ||
Revision as of 15:46, 10 September 2015
Curdlan synthase gene with codon optimisation for E. coli
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 678
Illegal XhoI site found at 400 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal AgeI site found at 1192 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Curdlan is a bacterial polysaccharide that has been of significant recent interest due to its interesting and valuable properties and its bioactivity.
In fact crdS codes Curdlan Synthase.
The crdS product (73 kDa) deduced from the DNA sequence (1,965 bp) is curdlan synthase. In Agrobacterium, CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a Nout–Cin disposition
The molecular genetics of curdlan production have been investigated in Agrobacterium sp. ATCC31749. Studies on transposon-insertion mutants with altered capacity to stain with aniline blue when grown on indicator agar identified four genes (crdA, crdS, crdC, crdR) that are essential for curdlan production
A central large and relatively hydrophilic cytoplasmic re- gion of !300 residues situated between TM3 and TM4 carries various conserved motifs, including the UDPGlc substrate-binding and catalytic D,D,D35QxxRW motif
The crdS gene of Agrobacterium sp. strain ATCC31749 encodes the curdlan synthase (CrdS) protein
encodes a membrane protein
Locus I is a putative operon of three genes in which the crdS gene, encoding a protein homologous with family GT- 2 glycosyl transferases (Coutinho and Henrissat, 1999), is flanked by the crdA and crdC genes (Stasinopoulos et al., 1999). The latter two genes encode proteins sharing no significant homology with reported protein sequences, and their roles in curdlan production are unknown.