Difference between revisions of "Part:BBa K1602005"
Line 16: Line 16:
 
         <br>
 
         <br>
 
         <br>
 
         <br>
−
        <img align="center" style="width: 565px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7b/Conversion_xylose_xylitol_v1.png">
+
<div align="center">
 +
        <img style="width: 565px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7b/Conversion_xylose_xylitol_v1.png">
 
         <p style="width: 900px; margin-left: 15px; margin-right: 15px;" alt="">
 
         <p style="width: 900px; margin-left: 15px; margin-right: 15px;" alt="">
 
             <b>Figure 1</b> Reaction scheme of the xylan to xylose converting operon. Xylose is the only substrate needed for the reaction. Xylose is metabolized to xylitol in 1 step in dependance of NADPH.
 
             <b>Figure 1</b> Reaction scheme of the xylan to xylose converting operon. Xylose is the only substrate needed for the reaction. Xylose is metabolized to xylitol in 1 step in dependance of NADPH.
 
         </p>
 
         </p>
 +
</div>
 
         <br>
 
         <br>
 
         <br>
 
         <br>

Revision as of 15:19, 10 September 2015

Xylose to xylitol converting operon

Xylose is a monosaccharide belonging to the aldopentose family. Through reduction it can be converted to xylitol. The reaction takes place in the cytosol of the host and recent studies show, that the formation of xylitol in E.coli seems possible as well.
To enable the reduction in E.coli it is mandatory to establish an operon containing the coding gene GRE3 for a aldose reductase. The gene is taken from Saccharomyces cerevisiae. The aldose reductase converts xylose to xylitol in dependence of NADPH.

Figure 1 Reaction scheme of the xylan to xylose converting operon. Xylose is the only substrate needed for the reaction. Xylose is metabolized to xylitol in 1 step in dependance of NADPH.



Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034) in front of it, under control of a T7 Promoter (BBa_I719005).


Figure 2 Genetic map of the xylose to xylitol converting operon with T7 promoter. This brick enables E.Coli BL21 cells to convert xylose to xylitol in presence of the inductor IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]